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KMID : 0368419930360030267
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Journal of Plant Biology
1993 Volume.36 No. 3 p.267 ~ p.273
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Differential Activation of Arabidopsis Cab Promoters during Greening of Transgenic Tobacco Shoots
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Lee, Choon Hwan/李春煥
Ko, Ye Jeong/Choi, Hong Keun/Rhew, Tae Hyong/Lee, Chin Bum/高禮貞/崔鴻根/柳兌馨/李鎭範
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Abstract
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Promoters of the chlorophyll a/b binding protein genes, cab1, and cab2, of Arabidopsis thaliana were studied for their functions in differential expression during greening of etiolated shoots. The etiolated shoots were derived from leaves of transgenic tobacco plants with the cab-CAT (chloramphenicol acetyltransferase) translational fusions, and CAT activity was measured to monitor the activities of the cab promoters. Cab1 promoter activity increased rapidly and showed saturation after about 24 hours of greening, but that of cab2 increased with about 2 day-lag period and showed saturation after 6 days, Cab1 promoter activity was more sensitive to levulinic acit (LA) compared with cab2 activity. Cab2 promoter activity was inhibited more sensitively by chloramphenicol (CAP) than by inhibitors of Chl formation. Cab1 promoter activity was, however, inhibited less sensitively by CAP than by LA. The treatment of abscisic acid (ABA) did not block Chl synthesis so significantly as LA treatment did, and cab2 promoter activity was much less sensitive to ABA compared with that of cab1. These results suggest that cab1 expression is strongly related with Chl formation, possibly with δ-aminolevulinic acid accumulation, and cab2 expression is suppressed more by the blockage of translation of Chl a-aporproteins than by the blockage of Chl a accumulation.
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